Generator
kanR

Part:BBa_P1003:Design

Designed by: Reshma Shetty   Group: BE.109 Instructors   (2006-01-23)

kanamycin resistance cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_P1003 is an kanamycin antibiotic resistance cassette. Several silent mutations have been introduced to eliminate useful restriction sites and rare codons. However, we inadvertently introduced a new BsmBI recognition site in BBa_P1003 (kanamycin antibiotic resistance marker) during the course of codon shuffling.

Source

References

<biblio>

  1. Watanabe-J-Bacteriol-1961 pmid=13783344
  2. Watanabe-Bacteriol-Rev-2003 pmid=13999115
  3. Anderson-Br-Med-J-1965 pmid=5321597

</biblio>